二苯乙烯苷对人乳腺癌T-47D细胞增殖及其雌激素受体表达的影响(1)
摘 要 目的：研究二苯乙烯苷(TSG)對人乳腺癌T-47D细胞增殖及其雌激素受体(ER)表达的影响，探讨其雌激素样作用及可能机制。方法：以ER阳性人乳腺癌T-47细胞为对象，以β-雌二醇(β-E2，1×10-8 mol/L)为阳性对照，采用CKK-8法检测不同浓度TSG(1×10-8、1×10-7、1×10-6、1×10-5、1×10-4 mol/L)作用24、48、72 h后的细胞增殖情况，并计算增殖率;分别采用Western blotting法和逆转录-聚合酶链式反应法检测经低、中、高浓度TSG(1×10-8、1×10-6、1×10-4 mol/L)作用48 h后细胞中ER-α、ER-β蛋白及其mRNA的表达情况。结果：经不同浓度TSG作用24、48、72 h后，各给药组各时间点(除β-E2组48 h外)的细胞增殖率均较空白组显著升高，且TSG 1×10-7、1×10-6、1×10-5 mol/L组显著高于β-E2组(P<0.05或P<0.01)。经低、中、高浓度TSG作用48 h后，各给药组细胞中ER-α、ER-β蛋白及其mRNA的表达量均较空白组显著升高(P<0.05或P<0.01);且TSG低浓度组ER-β蛋白的表达量，各浓度组细胞中ER-α mRNA以及低、高浓度组ER-β mRNA的表达量均显著高于β-E2组(P<0.05或P<0.01)。结论：TSG可诱导T-47D细胞的体外增殖，并可通过促进ER-α、ER-β蛋白及其mRNA的表达来发挥雌激素样作用;这种作用在一定浓度下强于β-E2。
关键词 二苯乙烯苷;植物雌激素;人乳腺癌T-47D细胞;细胞增殖;雌激素受体
ABSTRACT OBJECTIVE： To study the effects of stilbene glucoside (TSG) on the proliferation and estrogen receptor (ER) of human breast cancer T-47D cells， and to explore its estrogen-like effect and potential mechanism. METHODS： Taking ER positive human breast cancer T-47D cells as subjects， using β-estradiol (β-E2，1×10-8 mol/L) as positive control， CCK-8 assay was used to detect the cell proliferation after treated with different concentrations of TSG (1×10-8， 1×10-7， 1×10-6， 1×10-5， 1×10-4 mol/L) for 24， 48， 72 h; the cell proliferation rate was calculated. Western blotting assay and RT-PCR methods were adopted to detect the protein and mRNA expression of ER-α and ER-β in cells after treated with low， medium and high concentrations of TSG (1×10-8， 1×10-6， 1×10-4 mol/L) for 48 h. RESULTS： After treated with different concentrations of TSG for 24， 48， 72 h， the cell proliferation rate of each administration group at each time point (except for β-E2 group at 48 h) increased significantly， compared with blank group; those of TSG groups (1×10-5， 1×10-6， 1×10-7 mol/L) were significantly higher than β-E2 group (P<0.05 or P<0.01). After treated with low， medium and high concentrations of TSG for 48 h， protein and mRNA expression of ER-α and ER-β in cells were increased significantly， compared with blank group (P<0.05 or P<0.01); protein expression of ER-β in TSG low concentration group， mRNA expression of ER-α in TSG groups as well as mRNA expression of ER-β in TSG low and high concentration groups were significantly higher than β-E2 group (P<0.05 or P<0.01). CONCLUSIONS： TSG can induce the in vitro proliferation of T-47D cells and exert estrogen-like effects by promoting protein and mRNA expression of ER-α and ER-β， which is stronger than that of β-E2 at a certain concentration., http://www.100md.com(朱璨 王嫣 李尧锋 付云燕 唐文超 杨长福 王和生)
关键词 二苯乙烯苷;植物雌激素;人乳腺癌T-47D细胞;细胞增殖;雌激素受体
ABSTRACT OBJECTIVE： To study the effects of stilbene glucoside (TSG) on the proliferation and estrogen receptor (ER) of human breast cancer T-47D cells， and to explore its estrogen-like effect and potential mechanism. METHODS： Taking ER positive human breast cancer T-47D cells as subjects， using β-estradiol (β-E2，1×10-8 mol/L) as positive control， CCK-8 assay was used to detect the cell proliferation after treated with different concentrations of TSG (1×10-8， 1×10-7， 1×10-6， 1×10-5， 1×10-4 mol/L) for 24， 48， 72 h; the cell proliferation rate was calculated. Western blotting assay and RT-PCR methods were adopted to detect the protein and mRNA expression of ER-α and ER-β in cells after treated with low， medium and high concentrations of TSG (1×10-8， 1×10-6， 1×10-4 mol/L) for 48 h. RESULTS： After treated with different concentrations of TSG for 24， 48， 72 h， the cell proliferation rate of each administration group at each time point (except for β-E2 group at 48 h) increased significantly， compared with blank group; those of TSG groups (1×10-5， 1×10-6， 1×10-7 mol/L) were significantly higher than β-E2 group (P<0.05 or P<0.01). After treated with low， medium and high concentrations of TSG for 48 h， protein and mRNA expression of ER-α and ER-β in cells were increased significantly， compared with blank group (P<0.05 or P<0.01); protein expression of ER-β in TSG low concentration group， mRNA expression of ER-α in TSG groups as well as mRNA expression of ER-β in TSG low and high concentration groups were significantly higher than β-E2 group (P<0.05 or P<0.01). CONCLUSIONS： TSG can induce the in vitro proliferation of T-47D cells and exert estrogen-like effects by promoting protein and mRNA expression of ER-α and ER-β， which is stronger than that of β-E2 at a certain concentration., http://www.100md.com(朱璨 王嫣 李尧锋 付云燕 唐文超 杨长福 王和生)