蓝萼香茶菜的抗补体活性成分研究(5)
Chemical constituents of Rabdosia japonica var. glaucocalyx and
their anti-complementary activity
YAO Shi1， XU Nai-yu1， CHU Chun-jun1， ZHANG Jian1*， CHEN Dao-feng2*
(1.College of Pharmaceutical Science， Soochow University， Suzhou 215123， China；
2. School of Pharmacy， Fudan University， Shanghai 201203， China)
[Abstract] To study the chemical constituents of Rabdosia japonica var. glaucocalyx and their anti-complementary activity on the basis of preliminary studies. Target isolation guided by anti-complementary activity test， compounds in the chloroform and n-butanol fractions were isolated and purified by silica gel and Sephadex LH-20 column chromatographies， and preparative HPLC. The structures were identified by various spectroscopic data including ESI-MS， 1H-NMR and 13C-NMR data. The compounds were evaluated for anti-complementary activity in vitro. Eleven compounds were isolated from the chloroform and n-butanol soluble fractions and identified as stigmasterol (1)， stigmas-9(11)-en-3-ol (2)， glaucocalyxin D (3)， kamebakaurin (4)， maslinic acid (5)， corosolic acid (6)， minheryinsⅠ(7)， diosmetin (8)， caffeic acid ethylene ester (9)， caffeic acid (10) and vitexin (11). Isoquercetrin， rutin， quercetin， 3-methylquercetin， luteolin， 7-methylluteolin， and apigenin which were isolated from the preliminary studies together with compounds 9 and 10 showed inhibition of the complement system by the classical pathway. Compounds 2， 4， 6-9 and 11 were obtained from this plant for the first time. Caffeic acid (10) showed the strongest activity in vitro with a CH50 value of 0.041 g·L-1.
[Key words] Rabdosia japonica var. glaucocalyx； chemical constituents； caffeic acid ethylene ester； anti-complementary activity
doi：10.4268/cjcmm20130211
[责任编辑 孔晶晶], 百拇医药(姚士，徐乃玉，褚纯隽，张健，陈道峰)
their anti-complementary activity
YAO Shi1， XU Nai-yu1， CHU Chun-jun1， ZHANG Jian1*， CHEN Dao-feng2*
(1.College of Pharmaceutical Science， Soochow University， Suzhou 215123， China；
2. School of Pharmacy， Fudan University， Shanghai 201203， China)
[Abstract] To study the chemical constituents of Rabdosia japonica var. glaucocalyx and their anti-complementary activity on the basis of preliminary studies. Target isolation guided by anti-complementary activity test， compounds in the chloroform and n-butanol fractions were isolated and purified by silica gel and Sephadex LH-20 column chromatographies， and preparative HPLC. The structures were identified by various spectroscopic data including ESI-MS， 1H-NMR and 13C-NMR data. The compounds were evaluated for anti-complementary activity in vitro. Eleven compounds were isolated from the chloroform and n-butanol soluble fractions and identified as stigmasterol (1)， stigmas-9(11)-en-3-ol (2)， glaucocalyxin D (3)， kamebakaurin (4)， maslinic acid (5)， corosolic acid (6)， minheryinsⅠ(7)， diosmetin (8)， caffeic acid ethylene ester (9)， caffeic acid (10) and vitexin (11). Isoquercetrin， rutin， quercetin， 3-methylquercetin， luteolin， 7-methylluteolin， and apigenin which were isolated from the preliminary studies together with compounds 9 and 10 showed inhibition of the complement system by the classical pathway. Compounds 2， 4， 6-9 and 11 were obtained from this plant for the first time. Caffeic acid (10) showed the strongest activity in vitro with a CH50 value of 0.041 g·L-1.
[Key words] Rabdosia japonica var. glaucocalyx； chemical constituents； caffeic acid ethylene ester； anti-complementary activity
doi：10.4268/cjcmm20130211
[责任编辑 孔晶晶], 百拇医药(姚士，徐乃玉，褚纯隽，张健，陈道峰)