HPLC同时测定止喘灵口服液中氢溴酸东莨菪碱、硫酸阿托品、盐酸麻黄碱和盐酸伪麻黄碱的含量(3)
(1.Jiangsu Institute for Drug and Food Control， Nanjing 210008， China；
2. Jiangsu SZYY Group Pharmaceutical Limited， Jiangyan 225500， China)
[Abstract] Objective： To establish an HPLC method for determining the contents of scopolamine hydrobromide， atropine sulfate， ephedrine hydrochloride and pseudoephedrine hydrochloride in Zhichuanling oral liquid. Method： Agela Durashell RP-C18 (4.6 mm×250 mm， 5 μm) was adopted， with acetonitrile-sodium phosphate buffer solution (0.07 mol·L-1 sodium phosphate solution with 17.5 mmol·L-1 sodium dodecylsulfate adjusted to pH 6.0 with phosphoric acid solution) (30∶70) as the mobile phase. The flow rate was 0.9 mL·min-1， the detection wavelength was 207 nm， and the column temperature was 25 ℃. Result： Scopolamine hydrobromide， atropine sulfate， ephedrine hydrochloride and pseudoephedrine hydrochloride showed good linear relations with peak areas within the concentration range of 0.021 21-1.060 5 μg (r=0.999 3)， 0.011 14-0.557 μg (r=0.999 6)， 0.200 56-10.028 μg (r=0.999 7) and 0.070 33-3.516 5 μg (r=0.999 6)， respectively， with the average recoveries of 101.9%， 99.80%， 100.3%， 100.2% (n=6).Conclusion： The method was so quick， simple， highly reproducible and specific that it could be used as one of quality control methods of Zhichuanling oral liquid.
[Key words] Zhichuanling oral liquid； scopolamine hydrobromide； atropine sulfate； ephedrine hydrochloride； pseudoephedrine hydrochloride
doi：10.4268/cjcmm20131914, http://www.100md.com(张妤琳 李玉萍)
2. Jiangsu SZYY Group Pharmaceutical Limited， Jiangyan 225500， China)
[Abstract] Objective： To establish an HPLC method for determining the contents of scopolamine hydrobromide， atropine sulfate， ephedrine hydrochloride and pseudoephedrine hydrochloride in Zhichuanling oral liquid. Method： Agela Durashell RP-C18 (4.6 mm×250 mm， 5 μm) was adopted， with acetonitrile-sodium phosphate buffer solution (0.07 mol·L-1 sodium phosphate solution with 17.5 mmol·L-1 sodium dodecylsulfate adjusted to pH 6.0 with phosphoric acid solution) (30∶70) as the mobile phase. The flow rate was 0.9 mL·min-1， the detection wavelength was 207 nm， and the column temperature was 25 ℃. Result： Scopolamine hydrobromide， atropine sulfate， ephedrine hydrochloride and pseudoephedrine hydrochloride showed good linear relations with peak areas within the concentration range of 0.021 21-1.060 5 μg (r=0.999 3)， 0.011 14-0.557 μg (r=0.999 6)， 0.200 56-10.028 μg (r=0.999 7) and 0.070 33-3.516 5 μg (r=0.999 6)， respectively， with the average recoveries of 101.9%， 99.80%， 100.3%， 100.2% (n=6).Conclusion： The method was so quick， simple， highly reproducible and specific that it could be used as one of quality control methods of Zhichuanling oral liquid.
[Key words] Zhichuanling oral liquid； scopolamine hydrobromide； atropine sulfate； ephedrine hydrochloride； pseudoephedrine hydrochloride
doi：10.4268/cjcmm20131914, http://www.100md.com(张妤琳 李玉萍)