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温度、密度、喂食周期对菲牛蛭育苗影响的研究(1)
http://www.100md.com 2015年3月15日 中国中药杂志 2015年第6期
     [摘要]研究了温度,密度,喂食周期对菲牛蛭幼苗的生长规律。分别设置7 个温度(18,22,26,30,34,38 ℃和自然温度),5 个密度(75,125,200,275,350 条/L)和6 个喂食周期(2,4,6,8,12,16 d),经30 d的养殖后考察各处理的存活率,增长率,特定增长率等生长指标。特定生长率与温度之间存在二次曲线相关,其回归方程式为y=-0.066x/1+3.543 1x-38.09(R/1=0.837 9),当水温为26.84 ℃,菲牛蛭幼苗的特定增长率为最大9.461 4,菲牛蛭幼苗最适生长水温在26~30 ℃。水温为38 ℃时,菲牛蛭幼苗全部死亡;特定增长率与密度之间存在显著直线回归关系,其回归方程式为y=-0.005 7x+9.197 3(R/1=0.998 3);特定增长率与喂食周期之间存在显著直线回归关系,其回归方程式为y=-0.468 2x+10.574(R/1=0.998 8)。

    [关键词]菲牛蛭;幼苗;温度;密度;喂食周期;特定生长率
, 百拇医药
    Effect of water temperature, stocking density and feeding cycle on

    growth of juvenile Poecilobdella manillensis

    CHENG Bo-xing1,2, LIU Fei/1, GUO Qiao-sheng1*, SHI Hong-zhuan/1, LI Meng-meng/1, LU Yu-xi/1

    (1. Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China;

    2. Guizhou Normal College, Guiyang 550018, China)
, 百拇医药
    [Abstract]The effect of water temperature, stocking density and feeding cycle on the growth of Poecilobdella manillensis juvenile was conducted P. manillensis was conducted respectively under different conditions: water temperatures(18, 22, 26, 30,34, 38 ℃ and CT), stocking density(75, 125, 200, 275, 350 individual/L) and feeding cycle(2, 4, 6, 8, 12, 16 d). After 30 days, survival rate, weight gain rate, specific growth rate were measured. There was a significant correlation between water temperature and specific growth rate (y=-0.066x/1+3.543 1x-38.09, R/1=0.837 9). Based on the regression equation, the specific growth rate of P. manillensis achieved the maximum (9.461 4) at 26.84 ℃. And the most optimal water temperature was 26-30 ℃. Meanwhile, the survival rates of P. manillensis was 0 at 38 ℃ in 3 d. There was significant negative correlation between density and specific growth rate (y=-0.005 7x+9.197 3, R/1=0.998 3) and between feeding cycle and specific growth rate(y=-0.468 2x+10.574, R/1=0.998 8).
, 百拇医药
    [Key words]Poecilobdella manillensis; juvenile; temperature; stocking density; feeding cycle; specific growth rate

    doi:10.4268/cjcmm20150616

    菲牛蛭Poecilobdella manillensis Lesson为医蛭科牛蛭属动物,药材名金边蚂蟥,是广西特色的药用动物资源,收录于《广西壮族自治区壮药质量标准》第2卷(2011年版),又名堵平怀 [1]。菲牛蛭唾液腺分泌的天然水蛭素是目前世界上所发现的物质中较强的天然抗凝血酶物质,对治疗心脑血管疾病尤其是脑中风等有较高的疗效[2-4]。有关药用蛭类的研究主要集中在蚂蟥Whitmania pigra、水蛭Hirudo nipponia的动物生长习性[5-7]及遗传结构[8-10]等方面,对于菲牛蛭的研究则多集中在药理药效、抗凝血物质分离和提取等方面[11-13],关于菲牛蛭幼苗人工养殖的研究还未见报道。本试验从养殖温度、密度、喂食周期等3 个方面考察菲牛蛭幼苗的生长规律,以期为菲牛蛭的人工养殖提供科学依据。, 百拇医药(程搏幸 刘飞 郭巧生 史红专 李蒙蒙 卢昱希)
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