白头翁汤正丁醇提取物抑制白念珠菌细胞膜(1)
[摘要]該文探讨白头翁汤正丁醇提取物(butyl alcohol extract of Baitouweng decoction，BAEB)对白念珠菌细胞膜的抑制作用。以Spot assay观察BAEB对白念珠菌细胞活性的影响；酶标仪检测BAEB干预后白念珠菌细胞内渗透压变化；荧光显微镜观察BAEB对白念珠菌细胞膜通透性的影响；高效液相检测白念珠菌细胞膜麦角甾醇的含量；qRTPCR法检测细胞膜麦角甾醇生物合成相关基因的表达。结果显示，256，512，1 024 mg·L-1BAEB干预下白念珠菌活性逐渐降低；512，1 024 mg·L-1BAEB组白念珠菌胞内甘油含量显著性增多(P<005)，与白念珠菌胞内渗透压相关的基因HOG1分别下调91，93，55倍；512，1 024 mg·L-1BAEB组白念珠菌红色荧光细胞明显增多；1 024 mg·L-1BAEB干预后麦角甾醇峰面积为35884 95，有显著性差异(P<005)；1 024 mg·L-1BAEB干预组ERG1，ERG2，ERG3，ERG4，ERG5，ERG6，ERG10，ERG11，ERG13，ERG24，ERG25，ERG251，ERG26与UPC2 分别下调658，489，415，924，341，984，308，750，553，590，245，325，198，1007倍。BAEB可通过抑制麦角甾醇及其生物合成相关基因的表达，进而抑制白念珠菌细胞膜完整性。
, http://www.100md.com
[关键词]白头翁汤正丁醇提取物；白念珠菌；细胞膜；麦角甾醇
Butyl alcohol extract of Baitouweng decoction inhibits
Candida albicans cell membrane
HU Tangling 1，2， YUN Yun1，2， XU Zhiqing 1，2， DUAN Qiangjun 1，2， SHAO Jing 1，2，WANG Tianming 1，2， WANG Changzhong 1，2*
(1Institute of Integrated Traditional Chinese and Western Medicine， Anhui University of
Traditional Chinese Medicine， Hefei 230038， China；
, 百拇医药
2Institute of Integrated Traditional Chinese and Western Medicine， Anhui Academy of
Traditional Chinese Medicine， Hefei 230038， China)
[Abstract]To study the inhibitory effect of butyl alcohol extract of Baitouweng decoction(BAEB) on Candida albicans cell membrane The effects of BAEB on the activity of C albicans were observed by Spot assay The changes of intracellular osmotic pressure of C albicans after BAEB intervention were detected by microtiter plate reader The effect of BAEB on cell membrane permeability of C albicans were observed by fluorescence microscopy The content of ergosterol in C albicans cell membrane was detected by high performance liquid chromatography， and the expression of ergosterol biosynthesis related genes in cell membrane was detected by qRTPCR The results showed that the activity of C albicans was significantly decreased in 256， 512 and 1 024 mg·L-1 BAEB group The intracellular glycerol content of C albicans was significantly increased in 512 and 1 024 mg·L-1 BAEB group(P<005) The gene HOG1 associated with intracellular osmotic pressure of C albicans was downregulated by 91， 93 and 55 times， respectively C albicans with red fluorescent were increased significantly in 512 and 1 024 mg·L-1 BAEB group The peak area of ergosterol in the 1 024 mg·L-1 BAEB group was 35884 95， with a significant difference(P<005)； ERG1， ERG2， ERG3， ERG4， ERG5， ERG6， ERG10， ERG11， ERG13， ERG24， ERG25， ERG251， ERG26 and UPC2 were downregulated by 658， 489， 415， 924，341， 984， 308， 750， 553， 590， 245， 325，198 and 1007 times respectively in 1 024 mg·L-1 BAEB group The study indicated that BAEB could inhibit ergosterol and its biosynthesis related genes expression in the cell membrane and inhibit the activity of C. albicans., http://www.100md.com(胡唐玲云云徐志庆 段强军邵菁汪天明汪长中)
, http://www.100md.com
[关键词]白头翁汤正丁醇提取物；白念珠菌；细胞膜；麦角甾醇
Butyl alcohol extract of Baitouweng decoction inhibits
Candida albicans cell membrane
HU Tangling 1，2， YUN Yun1，2， XU Zhiqing 1，2， DUAN Qiangjun 1，2， SHAO Jing 1，2，WANG Tianming 1，2， WANG Changzhong 1，2*
(1Institute of Integrated Traditional Chinese and Western Medicine， Anhui University of
Traditional Chinese Medicine， Hefei 230038， China；
, 百拇医药
2Institute of Integrated Traditional Chinese and Western Medicine， Anhui Academy of
Traditional Chinese Medicine， Hefei 230038， China)
[Abstract]To study the inhibitory effect of butyl alcohol extract of Baitouweng decoction(BAEB) on Candida albicans cell membrane The effects of BAEB on the activity of C albicans were observed by Spot assay The changes of intracellular osmotic pressure of C albicans after BAEB intervention were detected by microtiter plate reader The effect of BAEB on cell membrane permeability of C albicans were observed by fluorescence microscopy The content of ergosterol in C albicans cell membrane was detected by high performance liquid chromatography， and the expression of ergosterol biosynthesis related genes in cell membrane was detected by qRTPCR The results showed that the activity of C albicans was significantly decreased in 256， 512 and 1 024 mg·L-1 BAEB group The intracellular glycerol content of C albicans was significantly increased in 512 and 1 024 mg·L-1 BAEB group(P<005) The gene HOG1 associated with intracellular osmotic pressure of C albicans was downregulated by 91， 93 and 55 times， respectively C albicans with red fluorescent were increased significantly in 512 and 1 024 mg·L-1 BAEB group The peak area of ergosterol in the 1 024 mg·L-1 BAEB group was 35884 95， with a significant difference(P<005)； ERG1， ERG2， ERG3， ERG4， ERG5， ERG6， ERG10， ERG11， ERG13， ERG24， ERG25， ERG251， ERG26 and UPC2 were downregulated by 658， 489， 415， 924，341， 984， 308， 750， 553， 590， 245， 325，198 and 1007 times respectively in 1 024 mg·L-1 BAEB group The study indicated that BAEB could inhibit ergosterol and its biosynthesis related genes expression in the cell membrane and inhibit the activity of C. albicans., http://www.100md.com(胡唐玲云云徐志庆 段强军邵菁汪天明汪长中)