乳酸和乳酸脱氢酶测定在不同类型脑膜炎中的鉴别作用分析
[摘要] 目的 探讨乳酸和乳酸脱氢酶测定在不同类型脑膜炎中的鉴别作用。 方法 选择80例患者，其中化脓性脑膜炎和病毒性脑膜炎各40例，在急性期和恢复期均行腰椎穿刺术后提取脑脊液2 mL，送检脑脊液常规、生化检查，比较患者脑脊液中乳酸和乳酸脱氢酶变化情况。 结果 化脓性脑膜炎和病毒性脑膜炎患者脑脊液乳酸脱氢酶在急性期显著高于恢复期(P < 0.05)，且化脓性脑膜炎患者脑脊液乳酸脱氢酶水平高于病毒性脑膜炎患者(P < 0.05)；化脓性脑膜炎患者脑脊液乳酸脱氢酶在急性期显著高于恢复期(P < 0.05)，且高于病毒性脑膜炎患者(P < 0.05)。结论 对不明类型的脑膜炎患者进行脑脊液乳酸和乳酸脱氢酶测定能起到一定的鉴别诊断作用。
[关键词] 乳酸；乳酸脱氢酶；化脓性脑膜炎；病毒性脑膜炎
[中图分类号] R446.14 [文献标识码] A [文章编号] 1674-4721(2012)07(c)-0128-02
, 百拇医药
Analysis of the effect of lactic acid and lactate dehydrogenase determination in different types of meningitis
YANG Wenxiu
Biochemistry Department of Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China
[Abstract] Objective To investigate the effect of lactic acid and lactate dehydrogenase determination in different types of meningitis. Methods Forty cases with purulent meningitis and 40 cases with viral meningitis were selected, and all patients were performed after lumbar puncture cerebrospinal fluid 2 mL in the acute stage and recovery stage. The cerebrospinal fluid routine, biochemical tests were checked; lactic acid and lactate dehydrogenase in cerebrospinal fluid were detected. Results Cerebrospinal fluid lactate dehydrogenase in acute period were significantly higher than the recovery period in purulent meningitis and viral meningitis (P < 0.05), and cerebrospinal fluid lactate dehydrogenase levels with purulent meningitis was higher than viral meningitis (P < 0.05), cerebrospinal fluid lactate dehydrogenase in acute period with purulent meningitis was significantly higher than recovery period (P < 0.05), and it was also higher than viral meningitis(P < 0.05). Conclusion Fluid lactic acid and lactate dehydrogenase assay can play a certain role in diagnosis of unknown type of meningitis cerebrospinal.
, 百拇医药
[Key words] Lactic Acid; Lactate Dehydrogenase; Purulent Meningitis; Viral Meningitis
早期化脓性脑膜炎及病毒性脑膜炎，其临床诊断和鉴别难度较大。实验室对于脑脊液的生化检测是一种行之有效的方法。本研究主要探讨脑脊液中乳酸的测定在化脓性脑膜炎于病毒性脑膜炎中的鉴别作用，现报道如下：
1 资料与方法
1.1 一般资料
选择2010年1月～2012年1月本科收治的化脓性脑膜炎和病毒性脑膜炎患者各40例。化脓性脑膜炎组中，男23例，女17例，年龄18～46岁，平均(31.6±3.8)岁。病毒性脑膜炎组中，男21例，女19例，年龄19～45岁，平均(32.0±3.4)岁。两组患者性别、年龄比较，差异无统计学意义(P > 0.05)，具有可比性。
, 百拇医药
1.2 方法
提取标本时将患者摆放侧卧胸膝位，将患者头脚尽量靠近，充分暴露脊柱，在急性期和恢复期均行腰椎穿刺术后提取脑脊液2 mL，送检脑脊液常规、生化检查。其中，脑脊液乳酸测定使用北京利德曼生化技术有限公司提供的酶显色法试剂盒(5011120)，同时使用奥林巴斯AU-2700全自动生化检测仪进行检测。
1.3 统计学处理
应用SPSS 13.0软件进行,计量资料以均数±标准差(x±s)表示，两组间均数比较用t检验，以P < 0.05为差异有统计学意义。
2 结果
2.1 两组患者不同时期脑脊液中乳酸变化情况
化脓性脑膜炎和病毒性脑膜炎患者脑脊液乳酸脱氢酶在急性期显著高于恢复期(P < 0.05)，且化脓性脑膜炎患者脑脊液乳酸脱氢酶水平高于病毒性脑膜炎患者(P < 0.05)。详见表1。, http://www.100md.com(杨文秀)
[关键词] 乳酸；乳酸脱氢酶；化脓性脑膜炎；病毒性脑膜炎
[中图分类号] R446.14 [文献标识码] A [文章编号] 1674-4721(2012)07(c)-0128-02
, 百拇医药
Analysis of the effect of lactic acid and lactate dehydrogenase determination in different types of meningitis
YANG Wenxiu
Biochemistry Department of Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China
[Abstract] Objective To investigate the effect of lactic acid and lactate dehydrogenase determination in different types of meningitis. Methods Forty cases with purulent meningitis and 40 cases with viral meningitis were selected, and all patients were performed after lumbar puncture cerebrospinal fluid 2 mL in the acute stage and recovery stage. The cerebrospinal fluid routine, biochemical tests were checked; lactic acid and lactate dehydrogenase in cerebrospinal fluid were detected. Results Cerebrospinal fluid lactate dehydrogenase in acute period were significantly higher than the recovery period in purulent meningitis and viral meningitis (P < 0.05), and cerebrospinal fluid lactate dehydrogenase levels with purulent meningitis was higher than viral meningitis (P < 0.05), cerebrospinal fluid lactate dehydrogenase in acute period with purulent meningitis was significantly higher than recovery period (P < 0.05), and it was also higher than viral meningitis(P < 0.05). Conclusion Fluid lactic acid and lactate dehydrogenase assay can play a certain role in diagnosis of unknown type of meningitis cerebrospinal.
, 百拇医药
[Key words] Lactic Acid; Lactate Dehydrogenase; Purulent Meningitis; Viral Meningitis
早期化脓性脑膜炎及病毒性脑膜炎，其临床诊断和鉴别难度较大。实验室对于脑脊液的生化检测是一种行之有效的方法。本研究主要探讨脑脊液中乳酸的测定在化脓性脑膜炎于病毒性脑膜炎中的鉴别作用，现报道如下：
1 资料与方法
1.1 一般资料
选择2010年1月～2012年1月本科收治的化脓性脑膜炎和病毒性脑膜炎患者各40例。化脓性脑膜炎组中，男23例，女17例，年龄18～46岁，平均(31.6±3.8)岁。病毒性脑膜炎组中，男21例，女19例，年龄19～45岁，平均(32.0±3.4)岁。两组患者性别、年龄比较，差异无统计学意义(P > 0.05)，具有可比性。
, 百拇医药
1.2 方法
提取标本时将患者摆放侧卧胸膝位，将患者头脚尽量靠近，充分暴露脊柱，在急性期和恢复期均行腰椎穿刺术后提取脑脊液2 mL，送检脑脊液常规、生化检查。其中，脑脊液乳酸测定使用北京利德曼生化技术有限公司提供的酶显色法试剂盒(5011120)，同时使用奥林巴斯AU-2700全自动生化检测仪进行检测。
1.3 统计学处理
应用SPSS 13.0软件进行,计量资料以均数±标准差(x±s)表示，两组间均数比较用t检验，以P < 0.05为差异有统计学意义。
2 结果
2.1 两组患者不同时期脑脊液中乳酸变化情况
化脓性脑膜炎和病毒性脑膜炎患者脑脊液乳酸脱氢酶在急性期显著高于恢复期(P < 0.05)，且化脓性脑膜炎患者脑脊液乳酸脱氢酶水平高于病毒性脑膜炎患者(P < 0.05)。详见表1。, http://www.100md.com(杨文秀)